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    • 专利摘要:
    Thienamycin related antibiotics PS-6 and PS-7 of the formula …<CHEM>… wherein R1 is CH3 and R2 is -CH2-CH2-, or R1 is H and R2 is -CH = CH-, and R3 represents hydrogen, lower alkyl or triphenyl methyl, and including the salts of the compound of formula (I) wherein R3 is hydrogen, said antibiotic substance having a strong antibiotic activity and a beta -lactamase inhibiting effect, and a method for producing the same by aerobic cultivation of Streptomyces A271 deposited with culture number FERM-P 3984 and ATCC number 31358.
    公开号:SU1039446A3
    申请号:SU782673547
    申请日:1978-10-12
    公开日:1983-08-30
    发明作者:Окамура Казухико；Хирата Содзи；Окумура Ясуси；Фукагава Ясуо；Симаути Ясутака；Исикура Томоюки；Коуно Кагеаки；Лейн Джозеф
    申请人:Санраку-Оушн Ко.,Лтд (Фирма)；
    IPC主号:
    专利说明:

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    This invention relates to the microbiological industry and concerns the production of antibiotics.  The purpose of the invention is to obtain an anti-tibiotic complex of the general formula J-Bg-LN- (0- (Hz dOOKj where, R-C-CH-CHj-), RjH antibiotic PS-6;, RI-H, (, - ant biotic PS-7.  This goal is achieved. According to the method for producing the antibiotic complex, Streptomyces strain L 271 is grown in a nutrient medium containing carbon, nitrogen and mineral sources. salt, under aerobic conditions at 2037 ° C and pH 5-9, followed by isolating the target product as an complex and dividing it into antibiotics PS-6 and PS-7 and / or translating them into salt, and / or translating antibiotics PS-6 and PS-7 and / or their salts in c denote, where Rj is lower alkyl or triphenylmethyl group.  In order to increase the release of antibiotic PS-6, valine and / or leucine or H-valeric acid are introduced into the nutrient medium in the culture proc.  The Streptomyces A 271 strain for obtaining an antibiotic complex was isolated from a soil sample near the Eygeji Temple in the Yoshida District in Fukui Prefecture, Japan.  The Streptomyces A 271 strain is deposited under the symbol FEPM-P No. 3984 in the Fermentation Research Institute (FRI), the Agency of Industrial Scienc and Tecnnology o Japan, and also the ATCC number No. 31538 in the America Type Culture Collection.  Morphological signs.  Branched sporulated aeral mycelium, the heads of the agral target are hooks, loops or incomplete spirals.  Oval or cylindrical spores form a chain of more than 10, usually 10-50 spores.  Size O ,, 01.01, 8 microns, the surface is smooth.  No flagella, no spur gi are observed.  On the aerial mycelium form from the hyphae.  Cultural features.  Saccharose nitrate agar medium.  Growth is plentiful, the color of aerial mycelium is bright orange-yellow.  Substrate mycelium is bright yellow to bright orange yellow.  Soluble pigment is missing.  Glucose-asparamnova agar medium.  Growth is plentiful.  Aerial mycelium is light orange yellow to bright orange yellow.  Substrate mycelium is bright yellow.  Soluble pigment is missing.   Glycerin-aspartic agar medium.  Growth is plentiful.  Aerial mycelium is light orange-yellow to bright orange-yellow, the substrate mycelium is pale yellow, slightly yellowish-clove. that  There is no soluble pigment.  Agar medium with starch.  Growth is plentiful.  Aerial mycelium is light orange yellow to bright orange.  yellow.  Substrate. Mycelium is bright orange yellow to bright yellow.  Soluble pigment is missing.  Nutrient agar medium.  Growth is plentiful.  Aerial mycelium is firmly formed, if formed, somewhat dark.  Substrate mycelium is light yellow.  Soluble pigment. not.  Agar medium with yeast malt extract.  Growth is plentiful.  Aerial mycelium is light orange yellow to bright orange yellow.  Substrate mycelium is bright orange to light brown.  Soluble pigment is missing. .  Agar medium with oat flour.  Growth is plentiful.  Aerial mycelium is light orange to orange-yellow, Substrate-mycelium yellow.  Soluble pigment is missing.  Physiological signs.  Grows at 10-40 ° C, the optimum growth of 20-30s.  Gelatin liquefies. .  Starch hydrolyzes.  Milk is paptonized, but does not coagulate.  Melanoid pigments do not form.  Well assimilates L-arabinose, D-xylose, D-glucose, L-ramnose.  Poorly absorbed sucrose.  Does not assimilate D-fructose, inositol, raffinose, D-mannitol. The method of obtaining the antibiotic complex is carried out by growing Streptomyces A 271 strain in a nutrient medium containing sources of carbon, nitrogen and mineral salts under aerobic conditions at 20-37 ° C.  Sources of carbohydrates are glucose, glycerin, maltose, sucrose, molasses, dextrin and starch, oils and fats, such as soybean oil, peanut oil, or lard.  Nitrogen sources can be peptone, malt extract, soybean flour, cotton seed oil, dry yeast, corn soaking liquid, yeast extract, skim milk, casein, sodium nitrate, ammonium nitrate, and ammonium sulfate.  Dikamit phosphate, sodium chloride, calcium carbonate, and magnesium sulfate are used as inorganic salts.  If necessary, trace elements may be added (cobalt and manganese).  Antifoaming agents, such as silicone or vegetable oils, may be added to prevent foaming during heat sterilization and fermentation.  In order to increase the yield of antibiotic PS-6, amino acids or organic acids can be added to the nutrient medium during the cultivation of Streptomyces A 271.  Neutral amino acids having 2-10 carbon atoms, in particular valine and leucine, can be used as an amino acid, and aliphatic organic acids having 2-10 carbon atoms, for example, can be used as amino acids.  measures N-valeric acid.  The amount of added amino acids or ortorganic acids can be in the range of 0.01-1% (w / v), preferably 0.1-0.5% (w / v).  These I compounds can be added at the beginning of cultivation or in the range of up to 100 hours from the start of cultivation.  Amino acids or organic acids can be added simultaneously or separately and supplied at various intervals.  Wednesday before cultivation can be sterilized.  pH 5-9.  Cultivation is performed under aerobic conditions.  The cultivation is carried out with stirring and / or aeration.   The growing temperature is 20-40 ° C, preferably 25-35 ° C.  Fermentation is carried out until a sufficient amount of PS-6 and / or PS-7 antibiotics is obtained.  The fermentation time is usually 30-90 hours, but it can vary depending on the composition of the medium and the temperature.  The amount of antibiotics PS-6 and / or PS-7 in the fermentation broth can be determined using the method of biological testing and bioautography. Since the obtained antibiotics PS-6 and PS-7 in the fermentation product are water soluble and are mainly present in the microbial cells, it is preferable to remove the cells after the fermentation process by such methods as filtration, centrifugation or extraction and remove the antibiotics from the filtrate of the supernatant layer or extract .  Removing antibiotics can be carried out using various known processes.  May be .  extraction at low pH with a solvent, e.g. ethyl acetate or n-butane, and back extraction from the solvent layer to the aqueous layer at higher pH, extraction at neutral pH.  a solvent such as methylene chloride or chloroform in the presence of lipophilic quaternary ammonium salts, such as benzalkonium chloride or tetrabutylammonium acid sulfate or cyclic compounds, such as dicyclohexyl-18-cyclo-6 ether, 15-cycle ether, and reverse extraction from lay  solvent in a neutral aqueous layer containing. sodium iodide, potassium iodide, adsorption on activated carbon Amberlite XAD, Dione HP-20 and elution with an aqueous solution of methanol, an aqueous acetone solution, adsorption and elution from an ion exchange resin such as Dowex 1x2 or GAE-Sephadex A-25, gel filtration with Sephadex g-10, Bio-Gel P-2, Bio-Beads Q-X3; Column or thin layer chromatography with cellulose Avicel 5F, DEAE cellulose.  Whatman DE-32, DEAE-Sephadex A-25, silica gel, oxide, “aluminum and precipitation when added, a scientific research institute of such solvent as acetone.  .  To isolate the antibiotic PS-7 from the antibiotic complex, having a saturated carbon chain in the sulfuric side chain, in which antibiotics PS-5 and PS-6 can be produced and obtained simultaneously, they are separated using conventional anion exchange chromatography on resins. wherein the active components are adsorbed on an anion-exchange resin of the plastic type, such as Dowex, Du6litA-101, Amberl11T 400 :. or Diaion PA 306, and antibiotics are eluted with an aqueous inorganic salt, followed by elution of the antibiotic PS-7 with an aqueous solution of a pyloric solvent, such as methanol or acetone with a concentration of 1-80, preferably 10-75%, containing 0.1-10, preferably 1- 5% inorganic salt such as sodium chloride, potassium chloride, calcium chloride, or sodium bromide.  Depending on the conditions of the stump: in the fermentation broth, the antibiotic IPS-4 or its salt is simultaneously formed.  Antibiotics PS-4: and PS-7 are produced simultaneously and can be separated from each other using various techniques, for example, adsorb a solution containing antibiotics PS-4 and PS-7 using an absorbent resin such as Diaton HP-20 AG , and antibiotics with a gradient of 0-50%, preferably 0710, are eluted. % polar solvent dissolved in water, such as an aqueous acetone solution.   Antibiotic PS-6 is characterized by the following physicochemical and biological properties.  Chromatography on paper.  Antibiotic PS-6 shows the following RI values for downstream paper chromatography in the following solvents: Acetonitrile Strys / EDTA) Ethanol (water 7/3 Thin layer chromatography (TLC For sodium salt of antibiotic PS-6 when thin-layer chromatography is performed using plate-like chromatograms 13254 and the following developing stretchers obtained R. J N-Butanol / Eta.  nol / water (4/1/5) (top layer) 0.67 N-Propanol / water (8/2) 0.69 N-Butanol / isopropanol / water (7/7/6) 0.63 Electrophoresis on paper with high voltage When PS-6 antibiotic (sodium salt) is subjected to electrophoresis on Toyo filter paper No. 50, it is indicative that the antibiotic migrates for a distance of at least 5 mm (usually 10-40 mm) towards the anode by passing a current in 30 minutes at a gradient of 42 V / cm in a buffer (pH 8.6) containing 3000 ml of water, 3.3 g of barbital and 25.5 g of barbital sodium.  Does not show activity on the basis of (Bacillus celreu 3-lactamase). The antibiotic PS-6 is a monobasic acid having one carboxylic group in the molecule.  The characteristic UV absorption maximum of the antibiotic PS-6 (sodium salt) is as follows: C | . ZOOH The characteristic absorption maxima of the IR spectrum of antibiotic PS-6 (sodium salt), measured by the method with the KBr tablet, -4760 (—Co in the lactam cycle); v 1660 (-CO-s yes); i; i600 (-СОО).  Molecular weight of approximately 312 (calculated from g results of high resolution mass spectrometry for methyl ester PS-6).  The reaction with Ehrlich reagent is positive.  The reaction with iodine-chloroplutinic acid is positive, the ninhydride reaction is negative.  PS-6 antibiotic is soluble in water at pH 6-9, but insoluble in benzene, acetone, and ethyl acetate.  Given the physico-chemical with.  properties clearly show that in the molecule of the antibiotic PS-6 (sodium salt) there are CHj-CH- groups; . - ™ - - - -M-NHCOI. c-oo, o According to the results of high-resolution mass spectrometry for methyl ester, the following p molecules were obtained {1a antibiotic formula PS-6: C / 4H oN204S.  The antibiotic PS-6 has the following structure:. . (Oylz o Biological properties of antibiotic PS-6.  The antibiotic PS-6, which has a broad spectrum of antibiotic activities, exhibits very high activity against various bacteria, for example, gram-positive bacteria belonging to the species Staphylococcus, Diplococcus, Streptococcus and Bacillus. and gram-negative bacteria belonging to the Aleaiigenes, Cbmamonas species.  Antibiotic PS-6 also shows. high activity, for example, against gram-negative bacteria belonging to the species Kiebsiella, Proteus.  Antibiotic PS-6 Shows high activity against gram-negative bacteria that are resistant to antibiotics, having a β-lactam cycle in the structure, for example, against those bacteria that belong to the species Citrobacter, Proteus, Kiebsiella, the antibiotic PS-6 has the ability increase the antibiotic activity of other antibiotics, especially β-lactam antibiotics, such as penicillins, cephalosporins, against such p-lactamise producing bacteria such as Pro-, teus yulgaris, Serratia marcescens.  In vivo activity.  The antibiotic exhibits a high therapeutic effect when administered to mice infected with pathogenic gram-positive bacteria. .  Toxicity.  The antibiotic is not toxic when administered intraperitoneally to mice at a dose of 500 mg / kg.  Antibiotics PS-7.  .  Physico-chemical properties of the antibiotic PS-7.  Chromatography on paper.  Antibiotic PS-7 (sodium salt shows the following Ri values for downstream chromatography on paper using Toyo paper filter paper No. 50 on the following solvent systems: Acetonite- ;; reel / tris / EDTA Ethanol / water Thin layer chromatography (TLC Antibiotic PS- 7 (sodium salt shows the following R / values at thin-layer chromatography on a 13254 cellulose chromatographic plate with the following solvent systems: N-Butanol / ethanol / water (4/1/5) (top layer) N-Propanol / water n-Butanol 6 / isopropanol / water C7 / 7/6) Acetonitrile / in yes (8/2) high voltage electrophoresis on paper.  The antibiotic PS-7 (sodium salt by electrophoresis on a Toyo filter paper No. 50 using high voltage electrophoresis equipment on paper migrates to the anode by at least -5 mm (usually in the range of 1040 mm) in a buffer solution containing 3, 3 g barbital, 25.5 g barb tala. sodium and 3000 ml of water (pH 8.6 when passing a current of 42 V / cm for 30 min.  The iS-7 antibiotic is inactive to Bacillus cere p-lactamase. The PS-7 antibiotic is a uniaxial acid containing one carboxyl group.  The UV absorption spectrum of antibiotic PS-7 (sodium salt) has the following characteristics (absorption maxima): I 220 nm and | . .  and the IR absorption spectrum of antibiotic PS-7 (sodium salt), measured. in a tablet with KBG, has characteristic absorption maxima with the following wave numbers ,.  cm: (—CO-R-lactam cycle); 4670 (-CO-amide); “-1620 (CO  and), Molecular weight of approximately 296 (calculated from high resolution mass spectrometry results for antibiotic methyl ester PS-7).  Reactions with Ehrlich reagent is positive.  The reaction with iodine-chloroplatinate is positive.  .  The reaction with ninhydrin is negative.  Let's easily dissolve in water at pH 9, practically insoluble in benzene, acetone and ethylamine.  The physicochemical properties lead to the statement that the antibiotic PS-7 (sodium salt) has the CH -CH- groups; SNOCO; 5f V-T.  -CH-lr / -NHCO-and-C00 in my; n-n pattern.  Antibiotic PS-7 is a compound with the following structural formula: CHj-CHi. -. ; -clH "lH-i H- f odH, oJ-to-Soon Biological properties of the antibiotic PS-7.  Antibiotic PS-7 has a broad spectrum of antimicrobial activity.  It shows very high antimicrobial activity on various microorganisms, for example, gram-positive bacteria belonging to the species Staphylococcus or Diplococcus, as well as high antimicrobial activity, in relation to gram-negative bacteria belonging, for example, to the species Alcaligenes.  An antibiotic is characterized by its high antimicrobial activity against gram-negative bacteria belonging, for example, to the species Citrobacter or to the species Enterobacter, which are resistant to the known |) -lactam antibiotics.  The antibiotic PS-7 has the ability to increase the antimicrobial activity of other antibiotics, for example, p-lacquer antibiotics, such as cephalosporins, against bacteria that produce (i-lactamase, such as Proteus vulgaris or Serratia marcescens.  Activity  The antibiotic has a significant therapeutic effect when administered to mice infected with pathogenic gram-positive bacteria.  The antibiotic does not show acute toxicity when administered intraperitoneally; mice at a dose of 500 mg / kg.  Antibiotics P. - C6 and PS-7 are stable. M: ; salt FormVSoleva,.  . f9 @ my.  includes, for example, alkali metal salts, such as sodium salt, potassium salt, or lithium salt.  -. alkaline earth metal salts, such as calcium or magnesium salts, salts of other metals, such as aluminum, ammonia, primary salts, in richic or tertiary amines, such as monoethylamine, dimethylamine, triethyl amine, monoethanolamine or diethylolamine, and organic salts such as like benzathine or procaine.  Alkali metal salts such as sodium or potassium salts are also suitable salts.  PS-6 antibiotics PS-7 are with monobasic acids having one carboxyl group and a lesion.  With the help of various alcohols of mercaptans or their derivatives from antibiotics, various esters can be obtained.  Suitable antibiotic esters PS-6 and PS-7 are those that respond to the following structural formula.  i. - -Hr-KH-eO-CH pj-G eoOKs1 where the lower alkyl group or triphenylmethyl group.  The lower alkyl group may be a straight or branched chain group, in particular a group having 6 or fewer carbon atoms, or a group that has 1-4 carbon atoms.  Examples are methyl, ethyl, n-propyl, isopropyl, n-butyl, -butyl, H-pentyl, t / z-pentyl and H-hexyl.  The esters of the above formula can be obtained by the interaction of the antibiotic PS-6 or PS-7 or their salts with compounds of the general formula.  where V is an atom or group that can be cleaved, or with lower diazoalkanes. Representative of compounds of this formula are methyl alcohol, methyl iodide, dimotilsulfonat, methyl mercaptan, chloride U-propyl iodide ff-propyl, propyl sleep iso-propyl alcohol, bromide, isopropyl iodide izoproil, n-Bu Tilova alcohol bromide, n-butyl, n-butyl iodide, n-pentyl m-pentyl sleep, n-pentyl methyl bromide, n-pentyl iodide; 1-H 5 ё1 her # 1-.  new alcohol, brmmiyt. y: n :. -l1 ekeyl, iodide N-hexyl, trityl spir ±, trityl mercaptan, trityl chloride and trityl bromide. ::.   The antibiotic reaction PS-6 or PS-7 with compounds of the formula or with lower diazoalkane can be carried out by known methods: esterification without a reaction medium, but usually it is carried out in an inert liquid medium.  Inert liquid media include, for example, hydrocarbons such as benzene, toluene, K-hexane or cyclo.  hexane, halogenated hydrocarbons, such as chloroform or chlorine, methylene, amides, such as dimethylformamide or hexamethylphosphorotriamide, dimethyl sulfoxide, ethers, such as diethyl ether, diisopropyl ether, AI-N-butyl ether, tetrahydrofluate, or tetrahydrochloride; such as ethyl acetate or H-butyl acetate, ketones, such as acetone or methyl ethyl ketone. The reaction temperature may vary widely, depending on the type of compound and the type of liquid inert medium, however, it must not exceed 60 ° C (preferably 0-40 ° C).  If necessary, a promoter such as trimethylamine, triethylamine, pyridine, or dicyclohexylcarbodiimide can be used in the reaction.  - Under these conditions, the reaction can be completed in 1-24 hours, usually in 3-12 hours.  Antibiotics PS-6 or PS-7, which should be. introduced into the reaction, optionally recovered as the product itself.  You can also use the raw antibiotic PS-6 or PS-7, obtained by partial purification of the cultivation product.  An example of such partially purified products is concentrated activated carbon eluate and concentrated eluate Dione HP-20, which process filtered broth, desalted concentrate of activated carbon eluate obtained with a concentration gradient of sodium chloride in phosphate buffer from CAE-Sephadex, which is adsorbed concentrated eluate from Diaion HP-20, concentrated methylene chloride extract in the presence of benzalkoniumchloidide, chloroform concentrated extract and butanol concentrated concentrate extract at pH 3.5 at low.  which temperature  The antibiotics PS-6 or PS-7 thus obtained can be isolated from the reaction mixture or obtained by known methods.  After completion of the reaction, the reaction cm is first poured into an aqueous medium to remove such water-soluble impurities. as byproducts.  Preferably, neutral buffers are used as the aqueous medium to maintain a neutral pH.  The antibiotic ester ester PS-6 or PS is then extracted into the mixture with a less polar organic growth solvent that is not substantially miscible with water, such as this acetate, benzene or chloroform.  Sodium chloride or ammonium sulfate may be added.  After drying over anhydrous sodium sulfate, the ester is isolated from the solvent layer by known methods, for example, gel filtration using Bio-Biids S-Xs or Sephadex LHr-20, or by adsorption chromatography using a silica gel, alumina or florosil.  The ester thus purified is purified by recrystallization from a solvent such as benzene, toluene, xylene, ethyl acetate, diethyl ether, methylene chloride chloroform, hexane, and petroleum ether.  Among the esters, the trityl ester of the antibiotic PS-6 or PS-7 is more stable than the antibiotic PS-6 or PS-7, and has a higher antibacterial activity and activity for inhibiting β-lactamase.  Physico-chemical and biological properties of trityl ester of antibiotics PS-6 and PS-7.  Physico-chemical properties of trit lovogo ester of antibiotic PS-6.  UV absorption spectrum; i J ° (315, 0 nm.  Color reactions.  The reaction with Ehrlich reagent is positive.  The reaction with iodine-chloroplatinic acid is positive.  Reactions with ninhydrin negative.  Thin-layer romatography (TLC) The following R | values were obtained on plates with the following solvents for those esters; DS-Fertigplatten Kieselgel 60 F Benzene / acetone (2/1) Benzene / ethyl acetate (1/8) Biological properties of tritiloetho antibiotic ester PS- 6  Antimicrobial spectrum.  The antibiotic trityl ester PS has a broad antimicrobial spectrum and is highly active against various bacteria, for example, gram-positive bacteria belonging to the species Staphyiococcus, Dipiococcus and Streptococcus; and gram negative bacteria. belonging to the species Alcaligenes.  The antibiotic trityl ester PS-6 also shows good activity against gram-negative bacteria belonging to the species Klebsieiia, Proteus.  The antibiotic trityl ester PS-6 exhibits high activity against gram-negative bacteria that are resistant to antibiotics that have a lactam cycle in their structure, for example, against bacteria of the species Citrobacter Proteus Klebsieiia and Serratia.  In vivo activity.  The antibiotic trityl ester PS-6 has a therapeutic effect when administered to mice infected with gram-positive pathogenic bacteria.  Toxicity.  . -. Trityl EF-IR is non-toxic when administered intraperitoneally to mice at a dose of 500 mg / kg.  The antibiotic trityl ester PS-7.  Physico-chemical properties of trityl ester of antibiotic PS-7.  UV Absorption Spectrum 321.0 nm 230.5 nm.  Color reactions.  The reaction with Ehrlich reagent is positive.  The reaction with iodine-chloroplatinic acid is positive.  Ninhydrin reaction is negative.  Thin layer chromatography (TLC).  DS-Fertigplatten Kieselgel 60 Fj. o -.   R, Benzene-acetone (2/1) Benzene-ethyl acetate (1/8) Biological properties of the antibiotic trityl ester PS-7.  Antimicrobial spectrum.  The antibiotic trityl ester PS-7 has a broad antimicrobial spectrum and exhibits very high activity against various bacteria, for example, gram-positive bacteria belonging to the species Staphyiococcus, Diplococcus and Streptococcus, and gram-negative bacteria belonging to the species L11111es.  -.  The antibiotic trityl ester PS-7 also shows good activity Against, for example, Gram-negative bacteria belonging to the Klebsieiia and Proteus species.  The antibiotic Trityl ester PS exerts high activity against gram-negative bacteria that are resistant to antibiotics having a β-lactam cycle in their structure, for example, against Citrobact Proteus, KlebsieJla and Serratia.  In vivo activity.  Trityl ester of antibiotic PS produces a high therapeutic effect when administered to mice infected with pathogenic gram-positive bacteria.  Toxicity.  Trityl ether does not exhibit acute toxicity when administered intraperitoneally in mice at a dose of 500 mg / kg.  The trityl esters of antibiotics PS-6 and PS-7 and the antibiotics themselves exhibit high antimicrobial activities and can be effectively used for the prevention and therapy of bacterial infections, which are not only in humans, but also in animals, for example mammals. in poultry and fish.  In addition, Trityl ester is very important as an intermediate for the synthesis of other pharmaceutical preparations, because the trityl group in the trityl ether is very active and easily cleaved.  Antibiotics PS-6 and PS-7 or their trityl esters can be administered orally, topically or parenterally (intravenously, intramuscularly,. intraperitoneally, etc. P. ) and can be used in one of the usual pharmaceutical preparations depending on the route of administration.  For example. , the antibiotic PS-6 tryl ester can be prepared with a pharmaceutically acceptable carrier or diluent in solid forms (for example, in the form of tablets, bowels, powders, granules, sugar-coated tablets, powders for spraying, candles), semi-solid forms (for example, ointments, creams, hard capsules) or liquid forms (for example, liquid solutions, emulsions, suspensions, lotions, syrups, injection solutions, liquid formulations for spraying).  A unit dose of a drug containing the antibiotic PS-6 or its trityl ether may contain 0.199 wt. .  %, (preferably 10-60) of the active component in any form: liquid, semi-solid or solid.  Tablets and capsules for oral administration may be in the form of a dosage unit and may contain binding agents, for example, syrup, gum, gelatin, sorbitol, tragekant or polyvinylpyrrolidone; fillers, such as lactose, sucrose, starch, calcium phosphate, sorbitol or glycine, lubricants, such as magnesium stearate, talc, polyethylene glycol, or silica, disintegrators, for example, potato starch; or wetting agents such as sodium lauryl sulfate.  Tablets can be coated with known SP-individuals.  Liquid preparations for oral administration can be in the form of oily or aqueous suspensions, solutions, emulsions, syrups, or can be released as dry products to be mixed. with water or other suitable carriers when used.  Liquid preparations for oral use may contain pharmaceutically acceptable adjuvants, such as, for example, suspending agents, for example, methylcellulose, sorbitol syrup, sugar or hydroxyethylcellulose, gelatin, carboxymethylcellulose, aluminum stearate gel, hydrogenated edible fats and oils; emulsifying agents, for example, gum, lecithin, sorbitan monooleate; non-aqueous carriers, for example ethyl alcohol, propylene glycol, are complex. oil esters, fractionated coconut oil, almond oil; and preservatives, for example methyl-P-hydroxybenzoate, propyl-P-hydroxybenzoate, sorbitol acid.  Candles may contain candle bases similar to coconut may and various glycerides.  Formulations for injection can be prepared in unit dosage form in ampoules or aqueous carriers, and, if necessary, they can contain formulating agents: suspending, dispersing and stabilizing.  The antibiotic can be prepared as a powder that can be reconstituted in pyrogen-free sterile water before use.  Compositions containing the antibiotic PS-6 or PS-7 and / or Li-3 antibiotics tertiary esters PS-6 or PS-7 can be prepared in various forms suitable for absorption through the mucous membranes of the nose, throat and bronchi, for example in powder form or sprayed liquid, inhalation drugs, tablets, throat lubricants.  For the treatment of ears and eyes, antibiotics can be prepared as individual capsules, in the form of drops, in liquid or semi-solid form; Mo, can also be represented as.
    formulations in hydrophilic or hydrophobic bases, such as powders, lotions, creams or ointments.
    In addition to the carrier, the compositions may contain other ingredients, for example, preservatives, antioxidants, lubricants, viscosity agents, perfuming agents, suspending agents, binders and stabilizing agents.
    Antibiotic PS-6 or PS-7 and / or. their trityl esters, intended for use in veterinary medicine, for example, for treating infections in pigs, cows, sheep, chickens, may be intrathoracic formulations in bases; long acting or fast releasing bases or in the form of concentrates for food additives.
    Pharmaceutical formulations may contain the antibiotic PS-6 or PS-7 Cu / or their trityl esters) as the sole active ingredient or in combination with other therapeutic effective
    ingredients.
    .one.
    : Since the antibiotic PS-6 or PS-7 and their trityl esters have a synergistic effect on various bacteria that produce -. p-lactamase in combination with lactam compounds, they are combined with (β-lactam compounds in pharmaceutical formulations; penicillin derivatives such as benzylpenicillium, phenoxymethylpenicillin, carbenicillin, amplicillin and amoxicillin; , such as cefaloridin, cefalotin, cefaolin, cefalexin, cefoxitin, cefastril, cefamandol, cefapirin, cefradine and cefaloglycine.
    The antibiotic PS-6 or PS-7 and / or their trityl esters are combined with one or more representative lactam compounds. It is desirable to use a quantitative ratio of the antibiotic to the known p-lactam compound (and m) in the range from 20: 1 to 1: 150, preferably from 10: 1 to 1: 100.
    When treating bacterial infections in humans, the dosage of antibiotic PS-6 or PS-7 and / or their trityl ester may vary depending on the object to be treated, body weight, type, tin and symptoms of infection, method and number of medications. . For normal oral or parenteral administration, it is convenient to use a daily dose in the range of 0.05-500 mg / kg, preferably 0.5-200 mg / kg, at a fractional dosage.
    The antibiotic PS-6 or PS-7 and / or their trityl esters can: - be used in pharmaceutical formulations or can be added directly or in the form of 5 concentrated food additives in food to animals. In addition, they can be used as food preservative ingredients. comrade or disinfectant. 0 Example. 500 ml of an Erlenmeyer flask containing seed culture medium (SE-4) is sterilized at 2 ° C for 15 minutes. To the well-sporulated oblique culture of Streptomyces sp. A 271, 10 ml of a 0.02% Tween-80 solution (surface active agent) is poured in and slightly mixed to form a spore suspension. Inoculate 1 ml of the spore suspension into a 500 ml Erlenmeyer flask and cultured with shaking for 48 hours on a rotating shaker (200 rpm, cycle radius 3.5 cm). Then, 2 ml of the seed culture in the 500 ml Erlenmeyer flask containing 100 ml of the next production medium and cultured with shaking for 48-96 h at 28 ° C on a rotating shaking device.
    Sowed culture. On Wednesday. (SE-4) weight / volume
    Beef extract,% 0.3
    5. Bacto tryptone, 0.5% glucose, 0.1% Soluble
    starch,% 2.4
    Yeast extract,% 0,5
    0 Calcium carbonate,% 0.4 Fat-free
    Soy flour,% 0.5
    pH before sterilization 7.5
    Production medium
    AG-1 Wednesday
    weight / volume
    0
    1.5
    Glucose,% Corn starch,%
    2.5 Liquid from soaking cuckoo,%
    2.0
    1.0 Dry yeast,% fl, L-MeTHOHHH,%
    OD CoCl26H20,%
    0.00013 pH before ster0
    7.2 lization
    ASA-2 Wednesday
    weight / volume Glucose,% 1.5 Potato
    2.5 starch,%
    5 Kuku2 soaking liquid, 0 Ruza,% 1.0 Dry yeast,% 0.00 CoCli- 6HgO,% AGB Medium w / v 3.0 Maltose,% Maize soaking liquid,% 1.0 Dry yeast,% CoCl2 % 0.00 pH before sterilization w / v AGB-41 medium Maltose,% 5.0 Soluble collapse 1.0 small,% Glycerin,% 0.3 Dry yeast,% 2.5 0.5 NaCi,%% 0.05 MgS04.,% 0.05 CaCO-,% 0.3 .CoCl eHjO,% 0.00 pH before sterilization w / v ML-19 Wednesday 4.0 Glycerin,% 0.5 Peptone,% Glucose,% 0.2 Potato starch,% Defatted soybean flour,% Dry yeast,% NaCl,% CaCO,% w / v AGO-1 medium Soybean oil,% Dry yeast,% NaCl,%,% MgS04-7H O,% CaCO- : i,% 0.00,% 7.0 pH before ste ilizatsiey Nogo A small amount of culture broth taken from the count for each sample at regular time Prome creepy. The active components in the samples are extracted, concentrated and the presence of anti-otics PS-6 and PS-7 is determined using chromium on paper and bioautography. Determination of antibiotic PS-6 Elute 10 ml of the supernatant from the centrifuged cultural broth in a minicolon holding 1, 5 ml Diaion HP-20. Upon absorption of the active component, it is eluted with an aqueous acetone solution, (50% v / v). Initially, O, 5 ml of the eluate is discarded, and 2 ml of the subsequent eluate is collected. The eluate is concentrated and 20 ml of concentrated eluate is subjected to descending paper chromatography using filter paper; it is developed at 5 ° C for 7-20 hours in various types of solvents. After the development, the still wet filter paper is attached to the plate for testing and for bioautography using various types of microorganisms for 15-30 minutes. Filter paper is then removed and the test plate is cultured at 30 ° C for 18 hours. The sample, including the antibiotic PS-6, forms the zone of inhibition at position R of the antibiotic PS-6. The antibiotic PS-6 B culture broths of the six media described are determined. Definition of antibiotic PS-7. A portion of 10 ml of the supernatant obtained by centrifuging the culture broth is placed in a minicolumn with 1.5 ml of Diaion PA-3040. The column is washed with a 0 .5 ml 5% (w / v) solution of sodium chloride and eluted with a 50% (w / v) methanolic aqueous solution containing 3% (w / v) sodium chloride. The first 1.0 ml of eluate is discarded and the next 5.0 ml of eluate is collected. After evaporation under reduced pressure to remove the methanol eluate. placed in a minicolumn with 1.5 ml of Diaion HP-20. The active component adsorbed by the resin is eluted with a 50% (v / v) acetone solution. The first 0.5 ml of the eluate is discarded, and the next 2.0 ml of the eluate is collected. The eluate is concentrated and 20 ml of the concentrate obtained is dripped as a spot on a Toyo filter paper No. 50. Downward chromatography is carried out on paper with various solvent systems at 5 ° C for 7-20 hours. After the half-dried eromatograms are developed, they are in contact for 15-30 minutes with the agar surface of the test plates for bio-biography, containing the various test microorganisms. Test plates are incubated at 35 ° C for 18 hours. A sample containing antibiotic PS-7 gives rise to an inhibition zone at the position of the antibiotic value PS-7. The results show that the antibiotic PS-7 is determined in all broth filtrates obtained using the above-mentioned four types of media, although their accumulated quantities differed from one another. Example 2. Streptomyces A271 is cultured according to the method of Example 1 with the exception that the previously mentioned AGB-42 media is used. 24 hours before the start and 24 hours after the start of cultivation, 0.2% D-valine or L-valine was added to the de. The amount of antibiotic PS-6 accumulated in the culture broth after shaking the tours for 72 hours is four times more than in the control culture broth, which valine did not add .. Content of the medium (AGB-42) - weight / v Maltose,% Soluble 1.0 starch,% 0.3 Glycerol,% 3.0 Dry yeast, NaCl,% 0.5 K2HP04,% 0.5 0.5 MgS04-TH O,% CaCOa,% 0, 3,% 0.00 pH before sterilization. Example 3. Streptomyces sp.A271 is cultured according to the method of example 1 with the exception of ML-19 medium described in above. 24 hours before the start and 24 hours after the start of cultivation, 0.2% Db-valine or L-linein was added to the medium. After 72 hours of cultivation upon shaking, the amount of PS-6 antibiotic accumulated in the broth is four times greater than in the control broth where valine was excluded. ; Example 4. Streptomyces sp. A271 according to the method of Example 3, but with the exception that D-leucine or L-lexing is added instead of D-or L-valine. After 72 hours of shaking the culture, the amount of antibiotic PS-6 accumulated in the broth is twice as large as in the culture broth from which leucine is excluded. Example 5. Streptomyces sp. A271 is cultivated according to the method of Example 1 with the exception that AGB-3 medium is used. Prior to culture, 0.1% L-valine and 0.3% L-leucine are added to the medium. After 72 hours of cultivation with shaking by the Scientific Research Institute of Vulgaris, a significant amount of the antibiotic PS-6 accumulates. The medium composition (AGB-3) w / v Maltose,% dry yeast, NaCl,% K2HP04,% MgSp4-7H20,%,% 0.3 eHjO,%; 0,00013 pH before st. 7.0. EXAMPLE 6 Cultivation was carried out according to the procedure of Example 2, but H-valeric acid was added instead of DL- or L-valine. The amount of antibiotic PS-6 accumulated in the broth under cultivation with shaking for 72 hours, i, is four times its amount in the control broth without the addition of H-valeric acid. Example 7. According to the method of Example 3, cultivation was carried out, but H-valeric acid was added instead of DL or L-valine. The amount of antibiotic. PS-6 accumulated in broth is twice as large as in control broth, to which n-valeric acid was not added. Example 8. Cultivation was carried out according to the procedure of Example 5. But n-valeric acid was added instead of L-valine. The amount of antibiotic PS-6 accumulated in the broth is four times more than in the control broth from which it was excluded. H-valeric acid. Example 9. Place 100 ml of a seed culture prepared as described in Example 1 into a 30 L fermenter containing: 15 liters of SE-4 medium, and culture with enhanced aeration at 28 ° C for 24 hours at 200 rpm The supply of sterile air is 7.5 l / h. Inoculate 1 liter of the medium in 100 l of AGB-42 medium (0.5% L-valine is added before inoculation) into a 200 l steel fermenter and cultured with enhanced aeration at 50 L / min at 28 ° C for 72 h at 100 rpm The antibiotic capacity of the optically hygroscopic supernatant of the broth is 450 CCE / ml. A 3% (w / v) perlite filter aid was added to the broth and filtered through a filter press, yielding 60 liters of filtrate (total antibiotic activity of 25.5-10b CCE). All subsequent operations are carried out under cooling conditions up to 6 ° C. The filtrate is adsorbed on a column packed with 6 l of ion-exchange resin, Diaion PA-306. The column was eluted with 20 L of a 5% (w / v) solution of sodium chloride to obtain the active fraction (A) containing antibiotics PS-5 and PS-6 (total antibiotic activity 4.2). The column after elution is used to isolate the antibiotic P8-7. The active fraction (A) is charged to colonic with Diaion HP-20 resin (cm), then eluted with 10% aqueous acetone solution. The eluate is concentrated under reduced pressure to distill off acetone and passed through a QAE-Sephadex column previously equilibrated to pH 8.0 with 1/100 phosphate buffer solution, eluted with a linear gradient of sodium chloride in M / 100 phosphate buffer a solution with a NaCl concentration of 1-0.4 M, the eluate is collected in fractions of 10 g. The collected fractions are diluted 100-fold and the active fractions are collected after determining the UV absorption spectrum and bioassay. In order to completely remove the antibiotic PS-5 contained in them, the active fraction is passed through a column with Dione HP-20 L and eluted with 300 ml of an aqueous solution of methanol with a linear gradient of methanol concentration of 10-75%. The volume of each fraction is 4 g. Each fraction is subjected to downward paper chromatography, developed with a mixed solvent consisting of 120 ml of acetonitrile, 30 ml (1/10 M Tris / oxymethyl-aminomethane-HCi buffer solution (pH 7.5 ) and 1 ml of 1/10 -M aqueous solution of sodium ethylenediaminetetraacetate (pH 7.5) and analyzed by bioautography.The active fractions from No. 25 to 30, containing the antibiotic PS-5, and the active fractions No. 33 to 40 containing the antibiotic PS -6, collected-. The active fraction containing antibiotic PS-6 is concentrated, and after distillation of methanol, the mixture is dried The lyophilized preparation thus obtained is dissolved in 1 ml of M / 100 phosphate buffer solution (pH 8.0) and then loaded for purification into a column (1.280 cm) with Sephadex G-10 and developed with the same buffer solution. the fractions were adjusted to pH 8.3 with a dilute aqueous solution of sodium hydroxide and then loaded onto a column (1.220 cm) with Dione HP-20. Elution was carried out with a 10% aqueous acetone solution for desalting. The active fraction is collected and freeze-dried to obtain 2.4 mg of a white lyophilized antibiotic preparation PS-6 as a sodium salt. A column of Dione, which is eluted with sodium chloride solution and stored to isolate antibiotic PS-7, is eluted with 50% (v / v) aqueous methanol containing 3% (w / v) sodium chloride to obtain the active fraction (B) (total antimicrobial activity is 1.1-10 of the EfE, yield 4.3%)
    The eluate of the active fraction (B) after concentration under reduced pressure to remove methanol is adsorbed on a column (370 cm) filled with Dione HP-20 and eluted with 10% (v / v) aqueous acetone. The eluate is fractionated into 25 g portions each.
    Combine the active fractions and concentrate under reduced pressure to remove acetone. The concentrate is adsorbed on a column (2 cm) with QAE-Sephadex, pre-aged in a buffer solution of k / lOOO phosphate buffer
    pH 8.0, and eluted with sodium chloride with a linear concentration gradient of 0.1-0.7 M in M / 100 phosphate buffer. The eluate is fractionated into 17 g portions each. Each fraction was analyzed by bioassay and paper chromatography followed by bioautography, and fractions nos. 25–33 were collected, combined, containing the antibiotic PS-7.
    The above active is adsorbed on a column (1.1 / 20 cm), filled with Diaion HP-20 LS, and eluted with acetone with a linear concentration gradient of 0-10% with a total volume of
    400 ml. The eluate is fractionated in portions of 5 g each. Each fraction is diluted 50 times for UV analysis. Collect fractions no. 15-20, which contain antibiotic
      lyophilized, get raw antibiotic drug PS-7.
    For further purification of the antibiotic PS-7, the lyophilized crude antibiotic preparation PS-7 thus obtained is dissolved in M / 100 phosphate buffer solution with a pH of 8.0 and loaded with Sephadex G10 and then. show the same buffer solution.
    The active fractions are combined and adsorbed on a column (1.1–20 cm) filled with QAE-Sephadex and pre-incubated in buffer M / 100 phosphate buffer with pH
    8.0, then eluted with sodium chloride at a linear concentration gradient of O — 0.4 M with a total volume of 200 ml. Combine the active fractions from the column. Sodium is added to the fraction with a concentration of 3% (w / w, volume). The solution is adsorbed on a column (1.1; 20 cm) filled with Dione HP-20AG, and the active ingredient is eluted with 10% (v / v) aqueous acetone and desalted. The active fraction was lyophilized to obtain 1.5 mg of antibiotic sodium salt PS-7 as a white lyophilized preparation.
    Similar procedures are repeated for
    obtaining drugs.
    The sodium salts of antibiotics PS-6 and PS-7 obtained in this way show the following physicochemical properties, respectively,
    I. Antibiotic PS-6.
    Antibiotic PS-6 is colorless, soluble in water and essentially insoluble in acetole.
    Decomposition point. When measured on a Kofler device for determining the melting point of VU-1 microquantities with increasing temperature at a rate of 1 ° C / min, the drug gradually turns brown and begins to decrease in volume at. About 220®C, the color of the preparation slowly changes - to brown - resin ..
    UV absorption spectrum.
    Dissolve the antibiotic PS-6 sodium salt in 3.0 ml of water and dissolve the spectrum on a spectrophotometer.
    .imo ° with 243.0 km and L, 0 them.
    A solution of hydroxylamine is added to an aqueous solution of this drug in distilled water. (pH 7.5) to achieve a concentration of PS-6 of 20 mg / ml and a concentration of the latter 10 mM. After 30 min at 22 ° C, the reaction mixture loses approximately. 95% of the original optical density at 300.0 nm.
    Color reactions.
    . Reactions with Ehrlich's reagent are beneficial. .
    The reaction with iodine-chloroplatinic acid is positive.
    Ninhydrin reaction is negative.
    Chromatography on paper.
    The antibiotic shows the following RJ values on a Toyo filter paper No. 50 with the following solvents for downward chromatography on paper:
    Kf
    0.41 0.65
    EDTA is a mixture of solvents consisting of 120 ml of acetonitrile, 30 ml of 1/10 M Tris Soxymethyl) -aminome-: tang-HC buffer solution (pH 7.5) and 1 ml of a 1/10 M aqueous solution of ethylene disodium sodium acetate (pH 7 ,five).
    Thin layer chromatography (TLC) ..
    The antibiotic PS-6 is subjected to chromatography on a sheet of 13254 cellulose with the indicated solvents, with the R.J. following:
    N-Butanol / ethanol / water (4/1/5),
    (top layer) 0.67
    0,69
    0.70 0.68
    High voltage electrophoresis on paper.
    0
    Antibiotic PS-6 is analyzed by electrophoresis on paper under high voltage.
    When conducting electrophoresis within 30 minutes after cooling (below 4 ° C) at a potential of 42 V / cm
    5 - In a buffer solution (pph of 8.6) containing 3.3 g of barbital and 25.5 g of barbital sodium salt in 3000 ml of water, antibiotic PS-6 is advanced 28 mm to the anode.
    0
    Antimicrobial properties.
    Minimum inhibitory MIC concentrations of antibiotic sodium salt were determined. PS-6
    5 on various pathogenic microorganisms, including resistant strains, using broth dilution techniques, using Brien Hiart Infusion Bros Aiken medium.
    The antibiotics PS-6 sodium salt preparation is dissolved in Brian Hiart Infusion Blow Aiken at a concentration in the range of 5-10 mg / ml, from which appropriate dilution series are prepared in the same liquid medium. Mic5 roorganisms are cultured for 18 h in Brian Hiart Infusion Bros Aiken at 28 ° C and inoculated into a diluted antibiotic series PS-6 with a final cell inoculum / ml. After standard
    0 cultures for 20 hours, record the growth of microorganisms in each dilution of antibiotic PS-6. The minimum inhibitory concentration (MIC) is
    The lowest concentration of the preparation of the antibiotic sodium salt PS-6, at which there is no visual confirmation of the reproduction of this microorganism. For control two
    0 known β-lactam antibiotic cefazorin and cefoxitin - dissolved in Brian Hiart Infusion Bros at pH 7.0 at concentrations in the range of 1-100 mg / ml; A series of several dilutions are prepared in the indicated liquid medium and treated as above for a specific MIC.
    The power and synergistic effect of the antimicrobial activity of known p-lactam compounds against p-lactam-resistant microorganisms.
    Sow 10 ml 2.5 times with diluted nutrient agar (pH 7.0) containing 50 mg / ml penicillin G
    or cephaloridine, B-lactamase-producing microorganisms and R-lactam-resistant microorganisms, and poured into 9 cm Petri dishes to provide an agar plate for bio-testing. A soft disk, 8 mm, containing 20 ml of the antibiotic PS-6 solution with a concentration of 240 mg / ml is placed on this plate and incubated at 35 for 18 hours before recording the zones of inhibition. A control plate for bio-testing is prepared in a similar way without penicillin G or cephaloridin. As standard antibiotics in the same conditions, 10,000 mg / mp of penicillin G — or 20 ml of cephaloridin — are applied to test disks.
    The results are shown in Table. one.
    Table 1
    5 Proteus vulgaris
    PS-614,0
    Penicillin G15,0
    Cefaloridin12,0
    PS-622,0
    Penicillin G 0.0 Cefaloridin 12.0 PS-6 20.0
    Serratia marcescens
    Penicillin G 0.0 S-18 Cefaloridin 11.0
    From the results given in table. 1, it can be seen that the addition of the antibiotic PS-6 to penicillin G or | cephal9ridine at a concentration below the defined limit clearly shows a synergistic effect against p-lactam-resistant microorganisms. The addition of penicillin G or cephaloridin instead of an antibiotic does not have a synergistic effect. In vivo activity. : Examine the therapeutic activity of the antibiotic PS-6 sodium salt preparation by administering to mice that were intraperitoneally infected with 5-10 cells / mouse with Staphylococcus aureus Smith. Shortly after infection, the preparation of antibiotic sodium salt PS-5 was injected subcutaneously. In DCU, male Shizuoka mice have a 50% therapeutic dose of this drug equal to 5.2 mg / kg.
    Toxicity.
    An aqueous solution for injecting the drug with sodium antibiotic PS-6 is intraperitoneally administered intraperitoneally to a male mouse (Shizuoka) with a dose of 500 mg / kg. Acute toxicity is not detected.
    28.5 15.0 12.0 2.0
    0.0
    12.0
    26.0
    0.0
    0.0
    11.0
    11.0
    Ii. Antibiotic PS-7.
    PS-7 antibiotic is soluble in water, but practically insoluble in acetone.
    The product does not have a definite melting point, it gradually softens at 145-150 ° C, shrinks in volume and looks solid with color at 220 ° C.
    UV Absorption I 220.0 nm and R j Q | ° j ,, 0 nm.
    Approximately 95% absorption at 308.0 nm is lost when an aqueous solution of hydroxylamine (pH 7.5) is added to deionize the aqueous solution of the product, the product concentration and hydroxylamine are adjusted in this way; about 20 mg / ml and 10 mM, respectively, the mixture is incubated for 30 minutes.
    Color reactions.
    Reactions with Ehrlich reativ is positive.
    The reaction with iodine-chloroplatinate is positive.
    Reactions with ninhydrin are negative.
    The antibiotic PS-7 gives the following 5 R values for downstream chromatographic on paper using Toyo filter paper No. 50 with the following solvent systems: Acetonitrile (Tris / EDTA) Ethanol / water (7/3) 0.68 Thin-layer chromatography (TLC Antibiotic PS-7 shows trace | RJ in TLC on chromatogram plates 13254 from cellulose (No. 6065) with the following system solvents: m-Butanol / ethanol / water (4/1/5) (top layer) 0, 65 N-Propanol / WATER (7/3) 0.81 N-Butanol / Isopropanol /: Doda (7/7/6) 0.71, Acetonitrile / water. Yes (8/2) -0.65 High electrophoresis paper on paper: See the following migration of PS-7 antibiotic to Toir with filter paper No. 50 into a buffer solution with the following pH values using high voltage electrophore equipment on paper: antibiotic PS-7 migrates to the anode for 28 mm in a buffer solution containing 3.3 g of barbital, 25.5 g of barbital of sodium, and 3000 ml of water, pH 8.6 at a voltage of 42 V / s for -30 min. Antimicrobial spectrum. Minimum inhibitory concentrations are determined on various pathogenic strains, including antibiotic-resistant PS-7 strains, using the Aiken medium dilution method. The antibiotic sodium salt of PS-7 is dissolved and diluted with Brown Hee Art Infusion Aiken medium at a concentration of 5-100 mg / ml. Next, the solution is diluted with the same medium for
    Antibiotic PS-7
    vulgaris p-5
    Penicillin G
    Cefaloridin
     iC-V
    19.0
    2; ,,. 15.0
    11.0 15.0
    12.0
    12.0 to obtain a series of geometric concentrations. A seed culture prepared by cultivation with shaking of various test microorganisms indicated in the Brian Hiart Infusion medium at 28 ° C for 18 hours was added to the diluted series, the final cell concentration being IlO ml. Cell growth is examined after incubation for 20 hours. The lowest concentration at which no cell growth is observed is the minimum inhibitory concentration of antibiotic PS-7 for the microorganism under test. 1 Power and synergistic effect on the antimicrobial activity of p-lactam antibiotics against p-lactam-resistant microorganisms. Cefaloridin is added to a 2.5-fold-diluted nutrient medium (pH 7, OJ and the p-lactate-producing strain is produced by the p-lactate-producing strain, which produces the p-oJ, and the r-lactoproductive strain that produces the r-lactate-producing strain, which produces the p-oJ, and the r-lacto-producing strain that produces the r-lactate-producing strain, which produces the p-oJ, and the r-lacto-producing strain that produces the r-lactate-producing strain, which produces the p-oJ, and the r-lactoproductive strain that produces the r-lactate-producing strain, which produces the p-oJ, and the anti-microbial activity of the p-lactate-producing strain that produces the p-lactate-producing nutrient medium (RJ) 10 ml of medium and from; stub in a Petri dish with a diameter of 9 cm to prepare a test plate. Dissolve the antibiotic PS-7 at concentrations of 50 and 29 mg / ml, respectively. Each 20 ml of solution and solutions diluted in two I-four In this case, a soft disk with a diameter of 8 mm is wetted. A soft disk is placed on the surface of the nutrient medium and the size of the inhibition zone near the disk is determined for 18 hours after the inhibition. As a control experiment, a similar experiment is carried out in a similar environment without adding cephaloridine. In addition, for comparison, a similar experiment was also conducted with a soft disk moistened with 20 ml of a solution of 10,000 mg / ml of penicillin G or cephaloridin instead of the antibiotic PS-7. The results are shown in Table. 2. Table 2
    -Lactam resistant
    Disc strain
    Antibiotic PS-7
    Citrobacter freundii E-9
    Penicillin G Cefaloridin
    Antibiotic PS-7
    Serrati-a marcescens S-18
    Penicillin G Cefaloridin
    The results table. 2 show that, in addition to the antibiotic PS-7, an apparent increase in antimicrobial activity is observed on the β-lactam-resistant strains of penicillin G or cephaloridine in such concentrations when no antimicrobial activity is shown without addition. When penicillin G or cephaloridine is added instead of the antibiotic PS-7, there is no increase in antimicrobial activity.
    In vivo activity.
    The activity of the obtained antibiotic PS-7 in mice infected with Staphylococcus aureus with 5-10 cells per mouse was determined intraperitoneally. The antibiotic sodium salt PS-7 NttjM was injected immediately after infection. The compound has a magnitude of., 5 mg / kg, respectively, in the male mouse DCU (Shizuoka).
    Toxicity.
    No acute toxicity was observed in male vtxasti (Shizuoka), which was intraperitoneally injected. The antibiotic sodium salt PS-7.
    Continuation of table 2
    The diameter of the zone of inhibition
    Additive cephNo supplements loridine
    13.5 0.0 0.0
    0.0
    0.0 12.0 12.0
    18.0
    11.0 0.0
    14.0
    0.0 0.0
    0.0
    0.0 11.0 11.0
    Example 10. Antibiotic trityl ester PS-6.
    25.9 mg of a white freshly dried antibiotic PS-6 preparation — sodium salt prepared according to the procedure described in Example 9 — is dissolved in 5 ml of dimethylformamide and 20 ml of triethylamine, and then 50 ml of trityl chloride are added. After stirring for 3 hours at room temperature, the reaction mixture is diluted with 200 ml of benzene and washed three times with 70 ml of 0.1 M phosphate buffer solution (fpH 6.8). After dehydration by adding 3 ml of a saturated aqueous solution of sodium chloride, the benzene solution is dried by adding anhydrous sodium sulfate. The benzene is distilled off under reduced pressure to obtain about 0.5 ml of benzene solution, which is passed through a column (1.2 x 96.0 cm) of Bio-Biids 5-X3. The column is developed with benzene. The active fractions obtained in this way are combined and concentrated to dryness under reduced pressure. The resulting residue is dissolved in O, 5 ml of acetone and loaded onto a column of t 1 / 296., O cm) with Sephadex LH-20, which is already swollen in acetone, the column is developing acetone. Combine the active fractions and concentrate to dryness under reduced pressure, to obtain 23.7 mg of a white powder. By recrystallization of this powder from a mixture of benzene and n-hexane, 17.5 mg of a colorless crystalline powder of trityl ester of antibiotic PS-6 are obtained. It is a colorless crystalline powder. . The UV absorption spectrum of antibiotic PS-6i trityl ester at a concentration of 100 mg / 3 ml of methanol and MeOH -e- - p. , I / iax V nm.4 Insoluble in water and H-hexane, highly soluble in benzene, ethyl acetate, chloroform, methanol, ethanol, acetone, dimethylformamide (DMF) and dimethyl sulfoxide (DMS Color reactions. Reaction with Ehrlich rea.ktivom polo Reactions with iodine-chloroplatinic acid are positive. Reactions with ninhydrin are negative. Thin-layer chromatography (TLC The migration site is assessed by bioautography on Comamorias terrigena B-996. those silica gel plates were applied and applied to a layer and put a layer and put a layer. acetone solution containing 10% g methyl form mida. Rf values are as follows: Benzene / ace-R # tone (2/1) 0.37 Benzoh / ethyl acetate (1/8) 0.34 Antimicrobial spectrum. The MIC values (minimum inhibitory concentrations) of the compounds are determined on various pathogenic organisms including several resistant strains using a dilution method in Vrain Hiar Infuzh Vro Aiken.Tityl ester of antibiotic PS is dissolved in a small amount of methanol and diluted as quickly as possible with Acryn pH 7.0 to trit concentration Lovogo ether antibiotic PS-6 in the range of 40-- 20 g / ml, the concentration of methanol in the final solution did not exceed 10%. The solution is diluted in twofold geometric series. Microorganisms are cultured for 18 hours at 28 ° C in Brian Hiart and Brought Aiken and inoculated with the indicated dilution series at the final cell inoculum / ml. The results were determined after incubation for 20 hours in 35 ° C. The values of minimum inhibitory concentrations (MIC) indicate the lowest concentration of units of tritiloyed antibiotic ester PS-6, when no growth of the corresponding microorganism is observed under the above conditions. The in vivo activity of the trityl antibiotic PS-6 was measured in mice that were intraperitoneally infected with 5 10 cells / membranes of StaphyJococcus aureus Smith. The tritylated antibiotic product PS-6 is administered subcutaneously almost immediately after infection. The 50% therapeutic dose for male DCCU mice (SHIZUOKA) is 5.5 mg / kg. Toxicity. No acute toxicity was observed in males DZU men (Shizuoka) with the administration of an intraperitoneal dose of the trityl ester of the antibiotic PS-6 500 mg / kg. Example 11. 24.5 mg of a white freshly dried preparation of antibiotic sodium salt PS-7, prepared as described in Example 9, are dissolved in 5 ml of dimethylformamide and 20 ml of triethylamine are added and then 50 mg of trityl chloride. After 3 hours. Stirring at room temperature, the reaction mixture is diluted with 200 ml of benzene and washed three times with 70 ml of O, 1 M pacTBOpai phosphate buffer (pH 6.8). After dehydration by adding 3 ml of a saturated aqueous solution of sodium chloride, the benzene solution is dried by adding anhydrous sodium sulfate. Benzene is partially distilled off under reduced pressure to obtain about 0.5 ml of benzene solution, which is then passed through a column (i; i., 0 cm) of BIO-BIID 5-X3. The column is developed with benzene. The active fractions thus obtained are combined and concentrated to dryness under reduced pressure. The resulting residue is dissolved in 0.5 ml of acetone and passed through a column (1.2. X96.0 cm) with Sephadex LH-20, which is already pre-swollen in acetone, and developed with acetone. Combine the active fractions and concentrate to dryness under reduced pressure to obtain 20.5 mg of the product as a white powder. Upon recrystallization of the Powder from a mixture of benzene with c-hexane, 15.5 mg of a colorless crystalline powder of trityl ester of the antibiotic PS-7 are obtained. It is a colorless crystalline powder. The UV absorption spectrum of the antibiotic PS-7 trityl ester is removed by 3 hours of the Double Biim Spectrophotometer Model 200-20 at a concentration of 100 mg / 3 ml of methanol. I 230, 5 321,5 them. The solubility of the tritylated antibiotic product PS-7 is determined by dissolving, with shaking, the Scientific Research Institute of 5 mg of each tritylated product of the antibiotic FS-7 in 0.1 ml of each of the following solvents at. The product is insoluble in water and W-reKcaHe, highly soluble in benzene, ethyl acetate, chloroform, methanol, ethanol, acetone, dimethylformamide (DMF) and dimethyl sulfoxide (DMSO). Color reactions. Reactions with Ehrlich reagent are positive. The reaction with iodine-chloroplatinic acid is positive. Reaction with ninhydrin negative, thin layer chromatography. Antibiotic trityl ester PS gives the following values under the specified conditions. The size of migration is determined by bioautography on a Sota monas terrigena B-996. A silica gel plate is used for those and, before applying the potassium, 2.5 ml of an acetone solution containing 10% dimethylformamide is dripped. The following Velin R is obtained in the following solvents Benzene / Acetone (2/1) 0.51 Benzene / Acetone (1/1) 0.67 Antimicrobial spectrum. Minimal inhibitory concentrations (MICs) are determined on various pathogenic organisms including some resistant strains using the Bryan Hiart Infusion Bros diluted culture Aiken method. Dissolve the trityl ester of the antibiotic PS-7 in a small amount of methanol and dilute as quickly as possible in Brian Hiart Infusion Bros Aiken medium (pH 7.0) to a concentration of trityl ether of the antibiotic PS-7 in the range of 40–20 mg / ml, and the concentration of methanol in the final solution does not exceed 10%. This solution is diluted in twofold, heated series. Microorganisms are cultured for 18 hours with Brian Hiart Infusion. Roll the Aiken and inoculate into the above series diluted at the final inoculum size of 1 x 10 cells / ml. Results are incubated after incubation at 35 ° C for 20 hours. The minimum inhibitory concentration means the lowest concentration of units of the trityl ester of antibiotic PS-7, at which no growth of the corresponding microorganism is observed under the conditions described. The antibiotic trityl ester PS-7 possesses a broad antimicrobial spectrum and, in particular, exhibits strong antibiotic activity against various (β-lactam-resistant (β-lactamase-producing) strains of microorganisms. In vivo activity. Antibiotic activity of the trityl ester of PS antibiotic 7 was measured in mice that were intraperitoneally infected with 5; lO cells / mouse with Staphylococcus aureus Smith. The antibiotic product PS-7 triturated was injected subcutaneously immediately after infection. 50% of the therapeutic dose in male SDU mice (Shizuoka) is 4, 5 mg / kg. No acute toxicity is observed in male DDU mice (Shizuoka) with the administration of PS-7 intraperitoneal trityl ester # but at a dose of 500 mg / kg. Example 12. Methyl eff. antibiotic PS-6. 2.0 g of lyophilized are suspended. of the preparation obtained in Example 9 in 1 ml of dry dimethylformamide (dMF) to which 20 ml of triethylamine and 50 ml of methyl iodide are added.After stirring the mixture for 2 hours at room temperature, about 20 ml of the mixture are taken, dissolved in methanol and Increased Absorption at 315 nm at Hitachi Recording Sp. trofotometre Model ER5-GP to pod.tverdit progress of the reaction. After confirmation, the entire reaction mixture is poured into 20 ml of benzene and immediately washed twice, each time with 10 ml of 0.1 M phosphate buffer solution (pH 7.0). The benzene solution is dehydrated over anhydrous sodium sulfate and concentrated to 1 ml at 35 ° C under reduced pressure. The concentrated solution was passed through a Bio-Biido S-X3 column (1.2 L iX85 cm) and developed with benzene. The eluate is developed by thin layer chromatography (Kieselgel 60 p254 benzene / acetone (1/1), collected after identifying the eluate fraction containing methyl ester of antibiotic PS-6, the identification is carried out using UV spectroscopy. The fractions are concentrated to dryness under reduced pressure, get a colorless oily compound, which is dissolved in 1 ml of acetone. The acetone solution is passed through a column of (1.2x85 cm) Sephadex LH-20, which was already variably swollen in acetone, and develop
    权利要求:
    Claims (2)
    [1]
    1. METHOD FOR PRODUCING AN ANTIBIOTIC COMPLEX of the general formula 'g-ZRr-ZNH-tfO-SER
    0H5 q J —- J — L (Jqqb 3 >
    where R 4 is SCC, Ri - (- CH 2 -CH * -), R, -H antibiotic PS-6;
    R ( -H p R 2 - (- CH = CH-), R.-H - antibiotic PS-7, characterized in that the strain Streptomyces A 271. grow in a nutrient medium containing sources of carbon, nitrogen and mineral salts, in aerobic conditions at 20-37 ° C and pH 5-9, followed by isolation of the target product in the form of a complex and its separation into antibiotics PS-6 and PS-7 and / or their translation into salts and / or translation of PS-6 antibiotics and PS-7 and / or their salts to the compound, where Rj is lower alkyl or triphenylmethyl group.
    [2]
    2. The method according to claim 1, I distinguish. and with the fact that, in order to increase the release of the antibiotic PS-6, into the culture medium c. Valine and / or leucine or H-valerianic acid are introduced into the cultivation process. · Priority by signs:
    '> 13.10.77 receiving antibiotic PS-6; 12/29/77 receiving antibiotic PS-7.
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    同族专利:
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    DK145382C|1983-04-05|
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    YU215182A|1983-01-21|
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    IT1099738B|1985-09-28|
    IT7828782D0|1978-10-13|
    YU42049B|1988-04-30|
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    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    US4235922A|1979-06-15|1980-11-25|Merck & Co., Inc.|3--6-ethyl-7-oxo-1-azabicyclo [3.2.0]hept-2-ene-2-carboxylic acid|EP0005349A1|1978-05-06|1979-11-14|Beecham Group Plc|Alkylcarbapenems, their preparation, use in pharmaceutical compositions and intermediates|
    EP0055990B1|1979-08-10|1986-06-11|Beecham Group Plc|Beta-lactam antibiotics, their preparation and use|
    US5213974A|1986-05-20|1993-05-25|Sankyo Company, Limited|Fermentation process for preparing antibiotics mureidomycins A, B, C and D|
    GB8928373D0|1989-12-15|1990-02-21|Erba Carlo Spa|Beta-lactam derivatives|
    US6287594B1|1998-01-20|2001-09-11|Edward S. Wilson|Oral liquid compositions|
    US20100069827A1|2008-09-12|2010-03-18|Barry Neil Silberg|Pre-Surgical Prophylactic Administration of Antibiotics and Therapeutic Agents|
    US9446227B2|2008-09-12|2016-09-20|Sonescence, Inc.|Ultrasonic dispersion of compositions in tissue|
    US9956388B2|2014-06-04|2018-05-01|Sonescence, Inc.|Systems and methods for therapeutic agent delivery|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    JP52121857A|JPS6215557B2|1977-10-13|1977-10-13|
    JP52160424A|JPS6213355B2|1977-12-29|1977-12-29|
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